Overall, the $30.01 billion bill would cut $539 million from the EPA compared to the fiscal 2015 enacted levels, for a total funding level of $7.6 billion. That's also well below President Obama's request of $8.6 billion.
The bill seeks to cut $75 million as well from EPA clean-air and clean-water programs and cuts $7.5 million from civil and criminal enforcement at the agency.
The bill passed by a voice vote, as is traditional in the Senate committee, and will face a full committee markup on Thursday.
The spending bill also looks to block several other landmark EPA rules, like the agency's clarification of its Clean Water Act authority. Republicans have long argued that the so-called Waters of the United States rule is a regulatory overreach and would give EPA too much power over agriculture and construction interests.
Another rider would bar the EPA from lowering the standard for ground-level ozone, or smog, until 85 percent of counties that currently do not meet the standard come into compliance. It would also block EPA from regulating lead fishing and tackle, and block a rule requiring companies to make financial plans to clean up hazardous-waste contaminations, which Democrats say would leave taxpayers on the hook.
Another rider in the bill would stop a White House guidance instructing federal agencies to consider climate-change impacts when they conduct National Environmental Policy Act reviews for major infrastructure projects.
Subcommittee Chairman Lisa Murkowski, R-Alaska, said the riders were designed to "rein in the EPA," adding that she was concerned the NEPA requirements would block construction projects.
But Democrats have said the policy language in the bill amounts to a list of poison pills that would keep them from supporting the Interior bill. Sen. Tom Udall of New Mexico, the ranking member on the panel, said he was "deeply disappointed" in the bill.
"What we see is nothing less than a backdoor attempt to rewrite the Clean Air Act" and other environmental laws, Udall said.
Among the other controversial riders is a provision that would block a Bureau of Land Management rule setting rules for hydraulic fracturing on public lands.
The bill would also block the U.S. Fish and Wildlife Service from enforcing an Endangered Species Act listing for the sage grouse, which Republicans have said would curtail oil and gas development in the West on land where the bird lives. The Interior Department has said it will go ahead with the determination process despite spending riders.
Overall, the bill would provide $11.05 billion for the Interior Department, $1.18 billion for BLM (a $65.5 million increase over fiscal 2015), and $2.73 billion for the National Park Service (a $112 million increase).
Heading into what's projected to be an above-average fire season, the bill also offers $3.61 billion overall to fight wildfires, reflecting the average amount spent over the last 10 years. The spending bill includes $1.05 billion in emergency spending and lifts the fire cap adjustment, ensuring that extra spending on wildfires does not come out of other federal programs.