A python, though firmly high on the food chain due to its size (up to 30 feet long), sometimes doesn't eat for a year or more. The snake has a peculiar biological mechanism to compensate for its sometimes prolonged fasting periods. After a meal, the mass of a python’s heart doubles within a day, and the triglycerides in its bloodstream increase fifty-fold. Its metabolism and production of insulin and lipids skyrocket.
Then, the python’s organs shrink back to their original size without losing newly generated muscle mass. A well-muscled heart allows the python to survive until the next unforeseen feast.
Leslie Leinwand, a molecular biologist at the University of Colorado at Boulder, began investigating this adaptation in 2005, dubbing her research team the Python Project. Leinwand and her team are looking into the potential implications of mimicking the organ growth, or hypertrophy, of the python.
When she told her colleagues she was ordering a crate of baby Burmese pythons for her lab, they thought she’d lost her mind. But she is not the first scientist to call for investigation in an increasingly popular area of science called “extreme biology” for clues to human health.
Jared Diamond, famous evolutionary biologist and geographer, highlights the python's organ growth in a 1998 Nature magazine article. Leinwand's lab shares data—and pythons—with Diamond’s colleague (and co-author on the Nature article) Stephen M. Secor of the University of Alabama. The snakes are rumored to glide freely around Secor’s lab floor.
According to the Centers for Disease Control and Prevention, heart disease is the leading cause of death in the United States. In 2005, the University of Colorado molecular biology research lab was working on defining what happens to the human heart as it gets bigger in instances of athletics, heart disease, genetic disease, and high blood pressure. Leinwand’s intent from the get-go was to derive a drug for curing and preventing heart disease by studying the pythons and their enlarged hearts.
The University of Colorado’s Molecular, Cellular and Developmental Biology research lab has long studied heart function. In 2013, Leinwand won the Bonfils Stanton Award in Science and Medicine for heart research.
“To determine what exactly is causing the enlargement of the python's heart we experimented by transfusing plasma from an [unfed] snake to another snake with no changes to speak of. Then we transfused plasma from a fed snake to a starved snake and its heart grew bigger,” Leinwand says.
The team identified three catalytic key fatty acids in the plasma of fed pythons: myristic acid, palmitic acid, and palmitoleic acid. This specific combination is the magic cocktail for tissue growth and a key discovery for the Python Project.
Dr. Cecilia Riquelme, an original team member of the Python Project, first called for experimentation to see the effect of python plasma on mammalian cells.
“I thought she would get discouraged. I thought it was too big of a leap.” Leinwand chuckles, remembering the conversation.
Riquelme didn't listen. She conducted an experiment in which she dropped snake blood on mice cells. Observing the cells’ reactions, she discovered the fatty acid combination indeed enlarges a mammalian heart.
Since the discovery the lab continues to test on healthy mice, pre-treating them with the discovered fatty acid combo, referring to it offhandedly as “snake oil.”
"The first step is to do no harm. And the effects so far have only been beneficial. By beneficial I mean the heart is larger morphologically, and geometrically. We can also look inside the heart to make sure we aren't triggering any disease pathways. We can measure that in a lot of different ways and we haven’t seen any evidence intracellularly that we are activating anything harmful," Leinwand says.
The next step is to determine if the fatty acid combination will reverse the progression of an already established heart disease.