Beyond being grossly overused and conjuring a horrendous massacre, it's not even technically accurate.
Before we get to the Kool-Aid part, let's recap some horrible American history. Jim Jones was a complex man. Long story short, he was a communist and occasional Methodist minister who founded his own pseudo-church in the late 1950s, called the Peoples Temple Full Gospel Church -- known in short as the Peoples Temple. (And yes, the omission of the possessive apostrophe is intentional, as the name apparently refers to peoples of the world.) While Jones called it a church, it was actually his version of a Marxist commune, with a smattering of Christian references thrown into his sermons/diatribes. The Peoples Temple was arguably a cult, demanding serious dedication (and financial support) from its members.
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As the Peoples Temple grew throughout the 1960s, Jones lost the plot on the whole Marxism thing and began to preach about an impending nuclear apocalypse. He even specified a date (July 15, 1967), and suggested that after the apocalypse, a socialist paradise would exist on Earth. And where would that new Eden be? Jones selected the remote town of Redwood Valley, California, and moved the Peoples Temple there prior to the deadline.
As you know, that end-of-the-world deadline came and went with no nuclear holocaust. In the following years, Jones abandoned all pretenses of Christianity and revealed himself to be an atheist who had simply used religion as a tool to legitimize his views. Jones said: "Those who remained drugged with the opiate of religion had to be brought to enlightenment -- socialism." Oh, and Jones was a drug addict, preferring literal opiates to metaphorical ones.
As media scrutiny increased and his political profile became more complicated, Jones became concerned that the Peoples Temple's tax-exempt religious status in the U.S. would eventually be revoked. He was also paranoid about the U.S. intelligence community. So in 1977, Jones again moved the Temple and its peoples, this time to a settlement he had been building since 1974 in the South American nation of Guyana. He named it "Jonestown," and it was not a nice place. It occupied nearly 4,000 acres, had poor soil and limited fresh water, was dramatically overcrowded, and Temple members were forced to work long hours. Jones figured his people could farm the land in this new utopia. It didn't hurt that he had amassed a multi-million-dollar fortune prior to arriving in Jonestown, though he did not share (or even use) the wealth. Jones himself lived in a small shared house with few luxuries.
U.S. Congressman Leo Ryan visited Jonestown in November of 1978, investigating allegations of human rights abuses within the Jonestown community. Ryan was accompanied by NBC News correspondent Don Harris, various other members of the media, and concerned family members of Jonestown residents. While visiting Jonestown, Congressman Ryan met a little over a dozen Temple members who wanted to leave (including a couple who passed a note reading in part, "Please help us get out of Jonestown" to news anchor Harris, mistaking him for Congressman Ryan). That number of defectors was actually quite low, considering the population of Jonestown, which was then over 900.
While processing paperwork to help Temple members return to the U.S., Ryan was attacked by knife-wielding Temple member Don Sly, but the would-be assassin was restrained before he could injure Ryan. Eventually the entire Ryan party plus the group of Jonestown defectors drove to a nearby airstrip and boarded planes, hoping to leave. But Jim Jones had sent armed Temple members (his creepily-named "Red Brigade") with the group, and the Red Brigade opened fire, killing Ryan, one Temple defector, and three members of the media -- and injuring eleven others. Those who survived fled into the jungle.
When the murderers returned to Jonestown and reported their actions, Jones promptly started up what he called a "White Night" meeting, inviting all Temple members. But this wasn't the first White Night. On various occasions prior to the murders, Jones had hosted White Night meetings in which he suggested that U.S. intelligence agencies would soon attack Jonestown; he had even staged fake attackers around Jonestown to add an air of pseudo-realism to the proceedings (though it's hard to imagine that such a small community wouldn't recognize their own people pretending to threaten the Temple). Faced with this hypothetical invasion scenario, Jones offered Temple members these choices: stay and fight the imaginary invaders, head for the USSR, head for the Guyana jungle, or commit "revolutionary suicide" (in other words, mass suicide as an act of political protest). On previous occasions when Temple members mock-voted for suicide, Jones tested them: Temple members were given small cups of liquid purportedly containing poison, and were asked to drink it. They did. After a while, Jones revealed that the liquid didn't contain poison -- but that one day it would. And, by the way, he had been stockpiling cyanide for years (not to mention piles of other drugs).
On the final White Night, Jones was not testing his Temple followers. He was killing them all.
After the airstrip murders outside Jonestown, Jim Jones ordered Temple members to create a fruity mix containing a cocktail of chemicals including cyanide, diazepam (aka Valium -- an anti-anxiety medication), promethazine (aka Phenergan -- a sedative), chloral hydrate (a sedative/hypnotic sometimes called "knockout drops"), and most interestingly... Flavor Aid -- a grape-flavored beverage similar to Kool-Aid. We'll get back to that last one in a moment.